ANALYSIS OF POLYMORPHISM OF MATRIX METALLOPROTEINASE-2 (C-1306 → T) AND TISSUE INHIBITORS OF METALLOPROTEINASE-2 (G303 → A) GENES IN PATIENTS WITH ANASTOMOTIC LEAK IN HOLLOW DIGESTIVE ORGANS

Abstract

The aim. To analyze the frequency of polymorphic variants of MMP-2 (C-1306 → T) and TIMP-2 (G303 → A) genes in patients with anastomotic leak in hollow digestive organs. The object of the study comprises 61 patients with anastomotic leak and connective tissue pathology, all treated at the Shalimov National Institute of Surgery and Transplantology during 2016-2019. Laboratory, genetic, histological studies and statistical analysis were performed. As a result of genetic and statistical analysis of the MMP- 2 (C-1306 → T) and TIMP-2 (G303 → A) gene polymorphisms, genotype variants have been identified that are associated with the risk of anastomotic leak in hollow digestive organs. Significant differences in the distribution of genotypes in the studied groups were revealed. Analysis of the multiplicative model of inheritance of MMP-2 and TIMP-2 genes showed compliance of genotype distribution with Hardy-Weinberg’s law. All models of inheritance were analyzed and the best model with the lowest Akaike Information Criterion, which turned out to be a recessive model, has been determined. Anastomotic leak in hollow digestive organs is 1.36 times more common in carriers of homozygous CC genotype of the MMP-2 gene and twice less common in minor homozygotes of TT (5.9% vs. 10%, p>0.05). It is statistically significant that in the group of patients with anastomotic leak in hollow digestive organs the GG variant of the TIMP-2 gene was detected 1.6 times more often. Carriers of minor homozygotes of AA genotype in the group with suture failure were not detected, while a similar genotype in the control group was found in 10% (p<0.05).