Definition of sensitization to pollen allergens wormwood and hazel in patients with respiratory allergic diseases by immunoblot and multiplex component test

Abstract

Specific diagnosis of respiratory allergic diseases is an indispensable component of allergology, which must be used at the stage of diagnostic search in a patient with allergopathology [1-3]. The place of each of these methods in the patient’s itinerary, however, is being actively discussed with the advent of new and new methods available for routine practice [4,5]. The aim of our study was to evaluate the diagnostic parameters of immunoblot and Immunocap ISAC methods to determine allergic sensitization to wormwood and hazel in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. The aim of our study was to evaluate the diagnostic parameters of immunoblot and Immunocap ISAC methods to determine allergic sensitization to wormwood and hazel in patients with respiratory allergic diseases – allergic rhinitis and bronchial asthma. Materials and methods. In this study, 40 patients with bronchial asthma and / or allergic rhinitis were examined with two different methods of specific allergic diagnosis (in vitro). The study was open-ended, comparative. Quantitative determination of specific IgE in the serum was performed using the RIDA® AllergyScreen immunoblot method (R-Biopharm AG, Germany) on the basis of the private laboratory of LLC “Allergy-Immunological Center KPP”. Immunocap ISAC testing was performed at “Forpost Allergy and Immunology Clinic”.