Polymorphism of matrix metalloproteinase-2 (C-1306→T) and tissue inhibitors of metalloproteinase-2 (G303→A) genes in patients with enterocutaneous fistula

Abstract

Aim: To analyze the frequency of polymorphic variants of matrix metalloproteinase-2(C-1306→ T) and tissue inhibitors of metalloproteinase-2(G303→ A) genes in patient’s with enterocutaneous fistula. Materials and methods: The object of the study comprises 63 patients with enterocutaneous fistula and connective tissue pathology who were treated in the Shalimov National Institute of Surgery and Transplantology during 2016-2019. Laboratory, genetic, histological studies and statistical analysis were performed. Results: As a result of genetic and statistical analysis of the matrix metalloproteinase-2(C-1306→T) and tissue inhibitors of metalloproteinase-2 (G303→A) gene single nucleotide polymorphisms, genotype variants have been identified that are associated with the risk of enterocutaneous fistula development. All models of inheritance were analyzed and the best model with the lowest Akaike information criterion, which turned out to be a recessive model, has been determined. Conclusions: Enterocutaneous fistula is 1,58 times more common in carriers of homozygous GG genotype of the tissue inhibitors of metalloproteinase-2(G303→A) gene and twice less common in heterozygotes GA (21.1% vs. 40%, p=0.057). Carriers of minor homozygotes of AA genotype in the group with enterocutaneous fistula were not detected, while a similar genotype in the control group was found in 10% of cases. It`s statistically significant that in the group of patients with enterocutaneous fistula the single nucleotide polymorphisms of the matrix metalloproteinase-2(C-1306→T) gene`s promoter doesn`t differ from the control group.